Facilities

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Laboratory: Research 3,576 sq. ft. Dr. Turner has a 900 sf ft main research laboratory and access to all support and specialized instrumentation spaces:: General Science Support Facilities: Autoclave and glasswashing room (200); Chemical Stock Room (350); Cryostorage (120); Drosophila culture laboratory (250); Biochemistry Research Laboratory (550); Biology Research Laboratory (1200); Tissue Culture Facility (320); Cold room (100); Dark room (64); Instrumentation Room (400); Fluorescence microscopy facility (120). CMB Prep Room (120 sq ft) houses storage, balances, microwave, dishwashing and other basic preparation equipment; Research Cold Room (100 sq ft); Research Dark Room (100 sq ft); Research Instrumentation Room (350sq ft); Imaging Core (120 sq ft and 400sq ft);  Biochemistry Prep Room; (100 sq ft); Autoclave Room (200 sq ft); Cryostorage Room (100 sq ft) All areas in square feet and in addition to approximately 10,000 sq. ft. of instructional laboratories. 

Office and Computers: Faculty members and the PI have 100 -200sq ft offices on the Chaminade campus. Each is equipped with a PC or MAC computers, printer and high speed wireless internet connection. Post-docs and graduate students have bench-adjacent desks in the research laboratory each equipped with a dedicated Mac Desktop and access to printing. Each investigator is equipped with either a PC or Macintosh computer purchased within the last three years. Laboratories are equipped with PC or Macintoshes that run equipment, and student write-up spaces have dedicated computers. High speed wireless internet and network printing are available throughout.

Equipment:

General.  Scottsman, Crushed Ice Maker (1), Gettinge, 4333LS Vacuum Steam Sterilizing Autoclave (1), Kenmore, Elite Dishwasher (3), Fisher Isotemp Benchtop Oven (1), Branson, Dual Bath Sonicator (1), Savant, ISS100 Integrated SpeedVac System (1), Lab-Line, Flammable Proof Refrigerators (3), Lab-Line, Flammable Proof Freezers – ½ size (6), VWR, (-80c) Super Freezer-24 cu ft (3).Tissue Culture: Dedicated tissue culture room (250 sq ft) with  2 Heraeus CO2 incubators and 3 (15 linear feet) flow hoods with HEPA filters and UV sterilization, vacuum pumps, tissue culture microscopes and refrigerated centrifuge. Liquid nitrogen cryostorage. Molecular Biology: Bio-Rad, Molecular Imager Gel Doc XR System (2), Bio-Rad, xMark Micorplate Absorbance Reader – monochromatic –all wavelengths (1), Eppendorf, Microcentrifuge 5424 (15), Nikon, Fluorescence Microscope with Mercury Halogen Light Source (1), Applied Biosystems PCR Machines (6), Thermo Spectronic, Genesys 2 (8 cuvette) UV Spectrophotometer (1), Thermo, Shaking/Orbiting Water Bath-10 gal (3), Brinkman, Bacteriological Incubator (2), MJ Research, PTC 225 Tetrad PCR Thermal Cycler (1), Bio-Rad, My iQ Real Time PCR Thermal Cycler with Single Dye Camera (1); GE Nanovue Spectrophotometer (1); Turner Biosystems Modulus Microplate Reader (1); GE Multiphor Electrophoresis system(1); multiple one and two dimensional protein electrophoresis and DNA electrophoresis systems; multiple Western blotting systems. Major chemistry instrumentation: Agilent LCMS 6100, Nicolet 380 FT-IR Spectrophotometer , GCxGC (Thermo fisher Trace 1300 Gas chromatograph and Thermo Fisher ISQ7000 Mass Spectrophotometer, SepSolve Analytical with INSIGHT™ flow modulator, ChromSpace GCxGC software, Markes Unity Xr Thermal Desorption unit, Thermofisher TriPlus RSH Robotic sample handling arm sampler). Protein chemistry and biochemistry: VWR, Orbital Shaker (2), VWR, Lab Rocking Table (2), GE Healthcare, Multiphor II 2-D Electrophoresis System with 3500XL Power Supply (1), Turner Biosystems, Veritas Luminometer Plate Reader (1), Olympus, BH-2 Fluorescence Microscope (1), Jenway, Genova UV Spectrophotometer-1 cuvette (1), GE Healthcare Nanovue UV Spectrophotometer (1), Barnstead, Labquake Tube Rocker (1), Hoefer Protein Gel Electrophoresis Systems (10), Bio-Rad, Mini-Protein III Protein Gel Electrophoresis System (20), Bio-Rad Western Blot Transfer System (20); Heidolph Laborata Rotavap (2); Flexidry MP Freeze Dryer (1). Electrophysiology: Faraday cage. Vibration isolating gas table. Nikon fluorescence microscope. HEKA EPC-10 USB patch clamp amplifier with head stage. Sutter P-1000 pipette puller. Eppendorf NP-2 Patchman manipulator. Eppendorf holding pipette manipulator. Automate Scientific 7 channel SmartSquirt perfusion system.  Patchmaster (2), Fitmaster (2) software and dongles. IgorPro software (2).Screening: FlexStation III (Molecular Devices). Imaging Core: Nikon Ti Eclipse EPifluorescent and C1 Laser Scanning confocal microscope system (1) with 3 solid state lasers, micropanipulation and microinjection system (Eppendorf), pressure controlled multichannel microapplicator systems (1 Eppendorf vario and 2 FemtoJet): aquisition and analysis computing stations (3) with full suite Nikon Elements and C1 software including digital deconvolution and multiple other analysis modules); Olympus BH-2 BHS PLM Microscope with video camera; (4) Four - Olympus BH-2 BHTU PLM student microscopes with digital imaging; Olympus BH-2  BHS vertical Illumination Brightfield/Darkfield microscope with digital imaging; Olympus SZ45 Stereomicroscope w/stand; Olympus BH-2 and BHA Phase Contrast Microscopes; Olympus BH-2 BHMJT Metallurgical Microscope with Nomarski DIC; Leica Transmission (Fiber) Comparison Microscope; Olympus BX51 TRF (transmission, reflection, fluorescence) Polarized Light Microscope (vertical brightfield, darkfield cubes, two fluorescence cubes in turret, 100W 12V Halogen Illuminator, Mercury Arc Illuminator, Xenon Arc Illuminator); Smith's Detection IlluminatIR micro-FT-IR (ARO, ATR) microprobe; Olympus BH-2  BHS Fiber Optic UV-Visible Microspectrophotometer; Olympus BH-2, UMA Vertical Illumination UV (Deuterium) microscope; Milton Roy Company, temperature controlled Abbe refractometor; Schwartz Fiber Microtome. Data Science and Bioinformatics Core. The university’s data science and analytics program is staffed by 4 faculty and housed in a 2000 sq ft purpose-renovated (2019) Data Science Center (DSC). The DSC comprises 3 faculty offices, a 24 student data science training center for data exploration and visualization (SAGE2 enabled cyberCANOE equipped classroom 82 Megapixels, 2 VR suites, VisLab high resolution tiled display with 298 Megapixels), accompanying storage, memory and processing capability. Total GPU capabilities:  18432 CUDA  Cores; 2304 Tensor Cores;  288 RT Cores;  and produces 56.8 TFLOPS of Single-Precision Performance and 456 TFLOPS  of Tensor Performance. The VisLab tiled display is the nucleus of a decision support and collaboration space with multiple work nodes. Data Science students and faculty work on client- and community-supplied use cases and problem sets, and specific faculty research interests include Cybersecurity/Human Factors, biologically-inspired data visualization for rapid decision-making, in silico design of metagenomic solutions for pollutant remediation, statistics and network theory for informatics and bioinformatic. To date there are two additional Data Visualization Laboratory (DVL) SAGE2 implementations at Chaminade. We implemented the CyberCANOE model pioneered by Dr. Jason Leigh (Laboratory of Advanced Visualization and Analytics, University of Hawaii, NSF ACI1441963, CNS1530873) SAGE2 comprises cloud-based and web-browser technologies to provide a framework for groups of students and researchers, whether in the room or remotely, to collaborate to solve problems that require viewing and controlling the display of large volumes of information in ultra high-resolution (SAGE2 website: sage2.sagecommons.org/project). Chaminade currently hosts two implementations, which differ only by the size of the displays: (1) Teaching Laboratory DVL consists of two 55 inch Sony Bravia 4K Ultra 3D grid-array monitors (18 sq. ft viewing area) (2) Lecture room DVL consists of two 65 inch LG 8500 4K Ultra HD(27.3 sq. ft display area).  SAGE2 software in the DVLs is run on Dell Area 51 computers with I7-6950X (10 core) CPU and two NVIDA GeForce GTX 1080Ti display cards, each with 11 GB of GDDR5X graphic RAM memory.  A 200 sq. ft Data Science Center is currently under renovation (opens May 2019) which will centralize all of these resources and add 6 Oculus VR-based visualization stations, 5 data analytics workstations and two new CyberCANOE data walls.